Moscow, Vavilova str., 43/5 (499) 135-99-57 info@ckpgene.ru
Home / Spectrophotometry / Sinergy 4

Synergy H4 Hybrid Multi-Mode Microplate Reader

Synergy™ H4 with Hybrid Technology™ is a patented multi-mode detector that combines the optical systems of Synergy Mx and Synergy 2 in one compact instrument. It is the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option. The monochromator-based optics provides a high level of flexibility; any wavelength can be used from the low UV to the near infrared. The quadruple grating optical system, equipped with a variable bandpass selection system, is ideal for spectral scanning applications. The filter-based optics use dichroic mirrors for enhanced performance. This system is faster and more sensitive than the monochromator optics. A dual reagent dispenser option is available for inject and read assays such as flash luminescent assays and fluorescent ion channel assays. Additional read modes include: fluorescence polarization, time resolved fluorescence and AlphaScreen/AlphaLISA.

Features:

  • Hybrid Technology: High-performance filter-based detection, flexible monochromator-based detection.
  • Detection Modes: Fluorescence intensity (FI), Fluorescence Polarization (FP), Time- Resolved Fluorescence (TRF), TR-FRET, High-Performance Luminescence, UV-Visible Absorbance, AlphaScreen / AlphaLISA.
  • Compatible with Take3™ Micro-Volume Platewith 2 μL microspots for low volume assays
  • Quadruple monochromator system and variable bandpass selection: Synergy H4 optics incorporates two double-grating monochromators. This quadruple design provides the best in spectral scanning performance and flexibility. Variable bandpass selection system adds flexibility.
  • Deep blocking filters and dichroic mirrors: Synergy H4’s filter/dichroic combination provides the best possible performance for fluorescence, time resolved fluorescence, fluorescence polarization and AlphaScreen / AlphaLISA applications.

Specifications:

General
Wavelength Selection Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141)
Quadruple Monochromators and Filters / Dichroics
Detection mode Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types Monochromator system 1- to 384-well plates
Filter system 1- to 1536-well plates
PCR plates
Compatible with Take3™ Multi-Volume Plate with 2 µL microspots
Temperature control 4° C above ambient to 65°, C + 0.5° C at 37° C
Shaking Yes
Software Gen5™ included; Control through USB or serial port
Automation Yes
AlphaScreen/AlphaLISA
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µ­L/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Absorbance
Light source Xenon Flash Lamp
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical <3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
Fluorescence Intensity
Sensitivity Monochromators:
Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom)
Filters/mirrors:
Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate)
Light source Tungsten Halogen Lamp
High Energy Xenon Flash Lamp
Wavelength selection Double grating monochromators (Top/Bottom) and,
Deep blocking filters / dichroic mirrors (Top)
Wavelength range Monochromators: 250 - 850 nm
Filters: 200 - 700 nm (850 nm option)
Dynamic range Monochromators: 5 decades
Filters/mirrors: >6 decades
Bandpass Monochromators: variable 9, 13.5, 17, 20 nm
Filters: filter-dependant, from 5 nm to >100 nm
Detection system Red shifted PMT for monochromator system
Low Noise PMT for filter-based system
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Luminescence
Sensitivity 10 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Fluorescence Polarization
Light source Tungsten Halogen
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Wavelength range 400 - 700 nm (320 - 850 nm option)
Time-Resolved Fluorescence
Light source High Energy Xenon Flash Lamp
Sensitivity Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Double grating monochromator (Top / Bottom)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Monochromators: 250 - 850 nm
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 78 lbs (35 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Methods and equipment

Spectrophotometry
Transfection
Polymerase chain reaction
Atomic force microscopy
Surface plasmon resonance
Nanotechnology