Moscow, Vavilova str., 43/5 (499) 135-99-57
Home / Spectrophotometry / Sinergy 4

Synergy H4 Hybrid Multi-Mode Microplate Reader

Synergy™ H4 with Hybrid Technology™ is a patented multi-mode detector that combines the optical systems of Synergy Mx and Synergy 2 in one compact instrument. It is the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option. The monochromator-based optics provides a high level of flexibility; any wavelength can be used from the low UV to the near infrared. The quadruple grating optical system, equipped with a variable bandpass selection system, is ideal for spectral scanning applications. The filter-based optics use dichroic mirrors for enhanced performance. This system is faster and more sensitive than the monochromator optics. A dual reagent dispenser option is available for inject and read assays such as flash luminescent assays and fluorescent ion channel assays. Additional read modes include: fluorescence polarization, time resolved fluorescence and AlphaScreen/AlphaLISA.


  • Hybrid Technology: High-performance filter-based detection, flexible monochromator-based detection.
  • Detection Modes: Fluorescence intensity (FI), Fluorescence Polarization (FP), Time- Resolved Fluorescence (TRF), TR-FRET, High-Performance Luminescence, UV-Visible Absorbance, AlphaScreen / AlphaLISA.
  • Compatible with Take3™ Micro-Volume Platewith 2 μL microspots for low volume assays
  • Quadruple monochromator system and variable bandpass selection: Synergy H4 optics incorporates two double-grating monochromators. This quadruple design provides the best in spectral scanning performance and flexibility. Variable bandpass selection system adds flexibility.
  • Deep blocking filters and dichroic mirrors: Synergy H4’s filter/dichroic combination provides the best possible performance for fluorescence, time resolved fluorescence, fluorescence polarization and AlphaScreen / AlphaLISA applications.


Wavelength Selection Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141)
Quadruple Monochromators and Filters / Dichroics
Detection mode Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types Monochromator system 1- to 384-well plates
Filter system 1- to 1536-well plates
PCR plates
Compatible with Take3™ Multi-Volume Plate with 2 µL microspots
Temperature control 4° C above ambient to 65°, C + 0.5° C at 37° C
Shaking Yes
Software Gen5™ included; Control through USB or serial port
Automation Yes
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µ­L/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Light source Xenon Flash Lamp
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical <3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
Fluorescence Intensity
Sensitivity Monochromators:
Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom)
Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate)
Light source Tungsten Halogen Lamp
High Energy Xenon Flash Lamp
Wavelength selection Double grating monochromators (Top/Bottom) and,
Deep blocking filters / dichroic mirrors (Top)
Wavelength range Monochromators: 250 - 850 nm
Filters: 200 - 700 nm (850 nm option)
Dynamic range Monochromators: 5 decades
Filters/mirrors: >6 decades
Bandpass Monochromators: variable 9, 13.5, 17, 20 nm
Filters: filter-dependant, from 5 nm to >100 nm
Detection system Red shifted PMT for monochromator system
Low Noise PMT for filter-based system
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Sensitivity 10 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Fluorescence Polarization
Light source Tungsten Halogen
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Wavelength range 400 - 700 nm (320 - 850 nm option)
Time-Resolved Fluorescence
Light source High Energy Xenon Flash Lamp
Sensitivity Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Wavelength selection Deep blocking filters / dichroic mirrors (Top)
Double grating monochromator (Top / Bottom)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Monochromators: 250 - 850 nm
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 78 lbs (35 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Methods and equipment

Polymerase chain reaction
Atomic force microscopy
Surface plasmon resonance