| General |
| Wavelength Selection |
Patented Hybrid Technology™ (protected under U.S. Patent 8,218,141)
Quadruple Monochromators and Filters / Dichroics |
| Detection mode |
Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
| Read method |
End point, kinetic, spectral scanning, well-area scanning |
| Microplate types |
Monochromator system 1- to 384-well plates
Filter system 1- to 1536-well plates
PCR plates
Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
| Temperature control |
4° C above ambient to 65°, C + 0.5° C at 37° C |
| Shaking |
Yes |
| Software |
Gen5™ included; Control through USB or serial port |
| Automation |
Yes |
| AlphaScreen/AlphaLISA |
| Light source |
Tungsten Halogen Lamp |
| Sensitivity |
100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
| Dynamic range |
>6 decades |
| Reading speed |
2 minutes typical for 96-well plate |
| Detection system |
Ultra Low Noise PMT |
| Absorbance |
| Light source |
Xenon Flash Lamp |
| Wavelength selection |
Monochromator |
| Wavelength range |
230 - 999 nm, 1 nm increment |
| Bandpass |
2 nm (230-285 nm), 4 nm (>285 nm) |
| Dynamic range |
0 - 4.0 OD |
| Resolution |
0.0001 OD |
| Pathlength correction |
Yes |
| Monochromator wavelength accuracy |
+2 nm |
| Monochromator wavelength repeatability |
+0.2 nm |
| OD accuracy |
<1% at 2.0 OD typical
<3% at 3.0 OD typical |
| OD linearity |
<1% from 0 to 3.0 OD typical |
| OD repeatability |
<0.5% at 2.0 OD typical |
| Stray light |
0.03% at 230 nm typical |
| Reading speed |
96: 11 seconds
384: 22 seconds |
| Fluorescence Intensity |
| Sensitivity |
Monochromators:
Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom)
Filters/mirrors:
Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate) |
| Light source |
Tungsten Halogen Lamp
High Energy Xenon Flash Lamp |
| Wavelength selection |
Double grating monochromators (Top/Bottom) and,
Deep blocking filters / dichroic mirrors (Top) |
| Wavelength range |
Monochromators: 250 - 850 nm
Filters: 200 - 700 nm (850 nm option) |
| Dynamic range |
Monochromators: 5 decades
Filters/mirrors: >6 decades |
| Bandpass |
Monochromators: variable 9, 13.5, 17, 20 nm
Filters: filter-dependant, from 5 nm to >100 nm |
| Detection system |
Red shifted PMT for monochromator system
Low Noise PMT for filter-based system |
| Reading speed |
96: 11 seconds
384: 22 seconds
1536: 42 seconds |
| Luminescence |
| Sensitivity |
10 amol ATP typical (flash) |
| Wavelength range |
300 - 700 nm |
| Dynamic range |
>6 decades |
| Fluorescence Polarization |
| Light source |
Tungsten Halogen |
| Sensitivity |
3 mP at 1 nM fluorescein typical |
| Wavelength selection |
Deep blocking filters / dichroic mirrors (Top) |
| Wavelength range |
400 - 700 nm (320 - 850 nm option) |
| Time-Resolved Fluorescence |
| Light source |
High Energy Xenon Flash Lamp |
| Sensitivity |
Europium 60 fM typical with filters (6 amol/well in 384-well plate) |
| Wavelength selection |
Deep blocking filters / dichroic mirrors (Top)
Double grating monochromator (Top / Bottom) |
| Wavelength range |
Filters: 200 - 700 nm (850 nm option)
Monochromators: 250 - 850 nm |
| Reagent Dispensers |
| Number |
2 syringe pumps |
| Dispense volume |
5 - 1000 µL in 1 µL increment |
| Dead volume |
1.1 mL, 100 µL with back flush |
| Physical Characteristics |
| Power |
250 Watts max |
| Dimensions |
17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) |
| Weight |
78 lbs (35 kg) |
| Regulatory |
| Regulatory |
CE and TUV marked. In Vitro Diagnostic use models are available. |
